Eluting & Discarding Renewable micro Column
3.4.6.
This clip simulates elution of an analyte from the column, by injecting an acid which turns the color of the pH indicator immobilized on the beads. As the zone of an acid passes through the column, the beads turn yellow and then dark again, when a basic carrier solution elutes the acid zone. Next, the column is discarded by means of rapid forward flow.
Note: Sepharose beads are elastic which allowed the use of forward flow for column removal. It is recommended to remove rigid beads should by flow reversal.
